Variability in Galactomannan detection by Platelia Aspergillus EIA™ according to the Aspergillus species.

نویسندگان

  • Melissa Orzechowski Xavier
  • Juliana S V Araujo
  • Valério Rodrigues Aquino
  • Cecília Bittencourt Severo
  • Luciana Silva Guazzelli
  • Luiz Carlos Severo
  • Alessandro Comarú Pasqualotto
چکیده

Here we investigate the extent to which different Aspergillus species release galactomannan (GM) in vitro. Marked variability was observed in GM reactivity between and within Aspergillus species, with A. terreus strains showing the highest GM indexes. The in vivo significance of these findings remains to be determined.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Galactomannan detection from piperacillin-tazobactam brands available in the Brazilian market.

Piperacillin-tazobactam is a broad spectrum antimicrobial agent that can cause false-positive results in the commercial Platelia Aspergillus EIA test. So far, no study has been performed in Latin America to evaluate the clinical implication of this finding. Here we studied the potential for galactomannan detection in piperacillin-tazobactam batches commercialized in the Brazilian market. Five b...

متن کامل

Diagnostic potential of nested PCR, galactomannan EIA, and beta-D-glucan for invasive aspergillosis in pediatric patients.

INTRODUCTION Limited specific data and investigations are available for invasive aspergillosis (IA) in pediatric patients. We evaluated the diagnostic potential of three noninvasive tests including the Platelia Aspergillus EIA kit for using galactomannan antigen, (1,3)-β-D-glucan Detection Reagent Kit, and nested-PCR for Aspergillus DNA in sera. We evaluated the diagnostic potential of three no...

متن کامل

Cross-reactivity of Fusarium spp. in the Aspergillus Galactomannan enzyme-linked immunosorbent assay.

Nine of 11 hematological patients with disseminated/deep-seated Fusarium infection tested at least twice for Aspergillus galactomannan (GM) had repeated positive results in the absence of Aspergillus isolation in culture. The centrifuged supernatants of 12 Fusarium isolates were tested by a GM enzyme-linked immunosorbent assay (EIA). All the isolates produced positive reactions when tested undi...

متن کامل

Using the galactomannan assay in the diagnosis of invasive aspergillosis.

Aspergillus species are ubiquitous molds to which humans are commonly exposed. Of approximately 180 species, it is estimated that 34 are medically significant.1 Most persons who come in contact with the fungus remain asymptomatic. However, some experience mild morbidity demonstrated by recurrent sinusitis, asthma exacerbations, or allergic bronchopulmonary aspergillosis. Patients who are immuno...

متن کامل

Assessment of Platelia Aspergillus enzyme immunoassay for the diagnosis of invasive aspergillosis.

BACKGROUND AND PURPOSE This study investigated the diagnostic value of Platelia Aspergillus enzyme immunoassay (EIA) for galactomannan (GM) antigen in patients at risk of invasive aspergillosis (IA), and its association with clinical course and outcome. METHODS A total of 304 blood samples were collected from 189 patients at risk of IA during a 1-year period at a tertiary referral center. Cla...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Revista do Instituto de Medicina Tropical de Sao Paulo

دوره 55 3  شماره 

صفحات  -

تاریخ انتشار 2013